Below are some frequently asked questions about our Antibody Production services. Should you have any questions other than listed below, feel free to contact us at antibody@premierbiochem.com and we will be happy to clear your thoughts.

1. What types of antibody services does PremierBiochem offer?

We offer both polyclonal and monoclonal services in rabbits and mice. For monoclonal projects we use mice, while for polyclonal projects, we use rabbits.

2. How are your antibodies purified, and what are your guarantees?

For polyclonal production, we offer both standard and guaranteed projects. Our standard projects use protein A or G purification and we guarantee the antibody will be dot-blot positive and have an ELISA titre >1:50,000. For our guaranteed projects, we use antigen affinity purification and in addition to our dot-blot and ELISA guarantee, we also guarantee that the antibody will work in your assay of choice.

3. Do you offer antigen production?

Yes, our experienced gene, peptide, and protein teams can produce your antigen in house either synthetically or in a recombinant expression system. Additionally, when ordering an antibody project, we offer these services at a reduced rate. Please inquire for details.

4. What are your antigen requirements?

If you are submitting your own antigen for immunization, the requirements vary depending on the project type. For protein antigens, we require 3mg protein (>85% purity) for our standard projects or 5mg protein (>85% purity) for our guaranteed projects. For peptide antigens, we require 20mg peptide (>85% purity).

5. Do you use a Carrier (hapten) when immunizing with a peptide antigen?

Yes, we will conjugate the peptide to KLH (keyhole limpet hemocyanin) as a carrier protein for injection with our peptide antigens.

6. Is it important to understand the end use application when considering antigen strategy?

Yes, it is very important to consider which assay your antibody will be used in. Not all antibodies work in all applications.
Western Blots recognize denatured proteins, while flow cytometry, sandwich ELISA, IF and other functional assays 
require antibodies that will recognize the natural, folded shape of the protein. 
A good understanding of your needs, along with antigen strategy, immunization strategy, screening strategy, 
purificationand validation can help to make a useful antibody.