Antibody Production

Anti-regular peptide polyclonal antibody

(customer-provided peptide)

1. Customer provides peptide: 1 mg non-conjugated; 2 mg KLH-conjugated (90% purity)
2. Immunization of two rabbits (other species available)
3. Perform test bleeds, titer tests
4. Purification of serum by protein A/G column
5. Antigen affinity purification may be obtained upon request

Anti-regular peptide polyclonal antibody

(we make peptide)

1. Design, synthesis and conjugation of peptide (15-20-mer, 5-10 mg)
2. Immunization of two rabbits (other species available)
3. Perform test bleeds, titer tests
4. Purification of serum by protein A/G column
5. Antigen affinity purification may be obtained upon request

1. Design, synthesis, and conjugation of phosphorylated peptide (15-20-mer, 5-10 mg)
2. Immunization of two rabbits (other species available)
3. Perform test bleeds, titer tests
4. Purification of serum by protein A/G column
5. Antigen affinity purification may be obtained upon request

1. Customer provides 5 mg protein antigen
2. Immunization of two rabbits (other species available)
3. Perform test bleeds, titer tests
4. Purification of serum by protein A/G column
5. Antigen affinity purification may be obtained upon request

10 mg purified antibody

Anti-regular peptide antibody 

(customer provide antigen)

1. Customer provides peptide (~4mg)
2. Immunization of up to five mice, fusion, screening, subclone and initial characterization
3. With up to three monoclones and their supernatant for confirming detection
4. Generated antibody recognizes immunogen in ELISA assay
5. Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid

Anti-regular peptide antibody 

(We provide antigen)

1. Design, synthesize and conjugate peptide (15-20mer,5-10mg)
2. Immunization of up to five mice, fusion, screening, subclone and initial characterization
3. With up to three monoclones and their supernatant for confirming detection
4. Generated antibody recognizes immunogen in ELISA assay
5. Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid

1. Design, synthesize, specific site phophorylation and conjugate peptide (11-15mer, 5 mg)
2. Immunization of up to five mice, fusion, screening, subclone and initial characterization
3. With up to three monoclones and their supernatant for confirming detection
4. Generated antibody recognizes immunogen in ELISA assay
5. Antibody purification by affinity column (1-5mg) may be obtained upon request

1. 4mg of purified Protein from customer
2. Immunize up to five mice, fusion, screening, subclone and initial characterization
3. With up to three monoclones and their supernatant for confirming detection
4. Generated antibody recognizes immunogen in ELISA assay
5. Antibody purification by affinity column (1-5mg) may be obtained upon request