Peptide Modifications

N-Terminus

Acetylation, Fmoc, Z(CBZ), Bz, Br-Ac-, Formylation,Fatty acid, Myristic acid, Palmytolyl, Suc, {HPP}, {Mpa} {pGlu}, DL-Lipoic acid, {Maleoyl-b-Ala}, Alloc, Boc, Stearic acid, {Maleoyl-b-Ala}, Alloc, Boc, Stearic acid

C-Terminus

Amidation, pNA, AMC, CMK, FMK, Cysteamide

C terminus ended amino acids: Ala, Gly, Met, Leu, Ile, Phe, Pro, Val

-OMe, -OEt, -OBzl, -OtBu, -TBzl, -NHMe, -NHEt, -NHisopen

C terminus ended amino acids: Lys, Gln, Asn, Cys, His, Arg, Ser, Thr, Tyr, Trp, Asp, Glu

-OMe, -OEt, -OBzl, -OtBu, -TBzl, -NHMe, -NHEt, -NHisopen

Special amino acid

{D-Ala}, {D-Leu}, {D-Met}, {D-Pro}, {D-Phe}, {D-Val}, {Hyp}, {Phg}, {D-Arg}, {D-Cys}, {D-Asp}, {D-Asn}, {D-Glu}, {D-Ser}, {D-His}, {D-Tyr}, {D-Thr}, {D-Trp}, {D-Lys}, {Abu}, {Aib}, {Nva}, {D-Nva}, {Nle}, {D-Nle}, {Cit}, {Orn}, {Lys(Ac)}, {Cys(Me)}, {Cys(Cam)}, {Cys(Acm)}, {Cys(tBu)}, {Hse}, {Hcy}, {D-Pen}, {Cha}, {D-Cha}, {Chg}, {D-Chg}, {D-1-Nal}, {L-1-Nal}, {D-2-Nal}, {L-2-Nal}, {D-2-Pal}, {L-2-Pal}, {D-3-Pal}, {L-3-Pal}, {D-4-Pal}, {L-4-Pal}, {D-4-Cl-Phe}, {L-4-Cl-Phe}, {D-4-F-Phe}, {L-4-F-Phe}, {D-4-NO2-Phe}, {L-4-NO2-Phe}, {D-4-I-Phe}, {L-4-I-Phe}, {Beta-Asp}, {Gamma-Glu}, {D-Ile}, {D-Orn}, {D-Gln}, {D-Cit}, {Dab}, {Dap}, {D-Beta-Asp}, {D-Gamma-Glu}, {Cpg}, {Pra}, {D-Pra}, {Gly(allyl)}, {D-Gly(allyl)}, {D-Hse}, {Pen}, {Har}, {HPh}, {Met(O)}, {Met(O)2}, {Tyr(Me)}, {4-NH2-Phe}, {Thr(Me)}, {Ser(Me)}, {Ac-Lys}, {D-4-NH2-Phe}, {Lys(Alloc)}, {Lys(Me2)}, {Lys(Dde)}, {Lys(Me)}, {Lys(Me3)}, {Ser(octanoic acid)}, {D-2-Me-Trp}, {Arg(Me)}, {Arg(Me)2} asymmetrical, {Arg(Me)2} symmetrical

SpacerLinker

{Mini-PEG}

{Ava}, C5

{Ahx}, C6

{GABA}, C4

{Beta-Ala}, C3

{Gly}, C2

N-Methyl amino acid

{N-Me-Ala}, {N-Me-Phe}, {N-Me-Leu}, {N-Me-Ile}, {N-Me-Val}, {N-Me-Met}, {N-Me-Nle},

{N-Me-Nva}, {N-Me-Gly}, {N-Me-Ser}, {N-Me-Tyr}, {N-Me-Thr}, {N-Me-Asp}, {N-Me-Glu}

Phosphorylation

{pSer}, {pTyr}, {pThr}, Di-sites in sequence, Tri-sites in sequence, {D-pSer}, {D-pTyr}, {D-pThr}

Fluorescence/Dye Labeling

Biotin (N-Terminal)

Biotin-LC (N-Terminal)

3-Indolylacetic acid (N-Terminal)

FITC (N-Terminal)

FITC-LC (N-Terminal)

5-FAM (N-Terminal)

5-FAM-LC (N-Terminal)

Dansyl (N-Terminal)

Dansyl-LC (N-Terminal)

MCA (N-Terminal)

TMR (N-Terminal)

Lys(Biotin) (middle)

Lys(Biotin) (C temrinus)

Lys(FITC) (middle)

Lys(5-FAM) (middle)

Lys(5-FAM) (C temrinus)

Lys(Dansyl) (middle)

Lys(Dansyl) (C temrinus)

Lys(TMR) (middle)

Lys(TMR) (C temrinus)

{Lys(Dnp)} (middle)

EDBiotin (C terminus)

EDDnp (C terminus)

{Lys(Maleimide)} (middle)

Lys(Pal) (middle)

Lys(Biotin) (N terminus)

Lys(Pal) (N-Terminus)

Lys(FITC) (N terminus)

Lys(5-FAM) (N-Terminus)

Lys(Dansyl) (N-Terminus)

Lys(TMR) (N-terminus)

Cyclic peptide

Mono Disulfide bridge

Double Disulfide bridge

Triple Disulfide bridge

Same Seq. Inter-Disulfide bridge

Different Inter-Disulfide bridge

Amide cyclic (end)

Amide cyclic (Side chain)

Quenched fluorescent peptide

Abz/ Tyr (3-NO2)

Abz

Tyr (3-NO2)

Glu(EDANS)-NH2

DABCYL

DABCYL/Glu(EDANS)-NH2

MAPS and Carrier Complex

Asymmetric 2 Branches (Pure)

Asymmetric 4 Branches (Crude)

Asym metric 8 Branches (Crude)

Glu 2 Branches (Pure)

n-mer Branch peptide on Lys side chain (Pure)

KLH-Peptide

BSA-Peptide

OVA-Peptide

Others

Aliquoting

N elemental Analysis

Solubility test

Water content (Karl Fischer)

Maldi-Tof MS

Switch salt